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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 89
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Cell_line_name |
72A1
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Brief_description |
confirmed as mouse with IEF of AST, MDH, PEP B established by fusion of the
P3X62AG8.653 mouse myeloma cell line with spleen cells of a BALB/c mouse
immunized with Epstein-Barr virus (EBV) particles; cells produce a monoclonal
antibody (IgG 1 ) that reacts with a viral envelope glycoprotein (350/220 kD) and that
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Description |
mouse hybridoma (anti-EBV) established by fusion of the P3X62AG8.653 mouse
myeloma cell line with spleen cells of a BALB/c mouse immunized with Epstein-Barr
virus (EBV) particles; cells produce a monoclonal antibody (IgG 1 ) that reacts with a
viral envelope glycoprotein (350/220 kD) and that neutralizes EBV confirmed as
mouse with IEF of AST, MDH, PEP B
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Depositor |
obtained from ATCC (HB 168), Rockville, Maryland, USA
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Morphology |
single cells in suspension
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Culture_conditions |
90% RPMI 1640 + 10% FBS maintain at 0.1-1.0 x 106 cells/ml; split every 2-3 days
at 1:3 to 1:5 at 37C with 5% CO2 1.0-1.5 x 106 cells/ml
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Viruses |
ELISA: reverse transcriptase positive; PCR: SMRV -
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Properties |
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule
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Mycolasma |
negative in DAPI, microbiological culture, RNA hybridization assays
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Bibliographic_reference |
Hoffman et al., Proc Natl Acad Sci USA, 77: 41 [PMID: 6248876
] |
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